Journal: Discover Oncology

Article Title: Clinacanthus nutans (Burm.f.) Lindau facilitates cuproptosis and ameliorates colon cancer progression by inhibiting PDE3B-mediated Apelin pathway

doi: 10.1007/s12672-025-02037-w

Figure Lengend Snippet: Clinacanthus nutans (Burm.f.) Lindau (CN) attenuates tumorigenicity of colon cancer in vivo. A The total ion chromatography of CN with top 10 chemical components labelled in the positive electrospray ionization modes. B The body weight curves of each group were recorded. C Statistical data of tumor weight in each group. D Statistical data of tumor volume in each group. E Representative pictures of mouse tumors in groups. F H&E staining of tissue sections. Amplification: 400 × , scale bar: 20 μm. G IHC analysis of Ki67 for tissues of xenograft tumors. Amplification: 400 × , scale bar: 20 μm. (H) VEGF, CD147 and MMP-9 protein expressions were detected by Western blot. ** p < 0.01, *** p < 0.001 vs. Colon cancer; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Low-CN

Article Snippet: Then the protein samples were separated on the sodium dodecyl sulfate polyacrylamide gel and electroblotted onto polyvinylidene fluoride membranes, which were then blocked with 5% skimmed milk for 2 h. These membranes were immunoblotted using primary antibodies against VEGF (ab32152, 1:1000, Abcam, Cambridge, UK), CD147 (13,287, 1:1000, Cell Signaling Technology, Danvers, MA, USA), MMP9 (ab283575, 1:1000, Abcam), PDE3B (ab99290, 1:1000, Abcam), LIAS (8641, 1:1000, Cell Signaling Technology), DLST (5556, 1:1000, Cell Signaling Technology), FDX1 (ab108257, 1:1000, Abcam), and GAPDH (5174, 1:1000, Cell Signaling Technology) at 4 °C overnight.

Techniques: In Vivo, Ion Chromatography, Staining, Amplification, Western Blot